2015-04-30

A smartphone with ultimate macro feature: DNA-scanning fluorescent microscope -- ScienceDaily

A smartphone with ultimate macro feature: DNA-scanning fluorescent microscope -- ScienceDaily

"A single DNA molecule, once stretched, is about two nanometers in width," said Aydogan Ozcan, HHMI Chancellor Professor, UCLA. "For perspective, that makes DNA about 50,000 times thinner than a human hair. Currently, imaging single DNA molecules requires bulky, expensive optical microscopy tools, which are mostly confined to advanced laboratory settings. In comparison, the components for my device are significantly less expensive."

Enter Ozcan's smartphone attachment -- an external lens, thin-film interference filter, miniature dovetail stage mount for making fine alignments, and a laser diode, all enclosed in a small, 3D-printed case and integrated to act just like a fluorescence microscope.

Although other smart-phone-turned-microscopes can image larger scale objects such as cells, Ozcan's group's latest mobile-phone optical attachment is the first to image and size the slim strand of a single DNA molecule.

The device is intended for use in remote laboratory settings to diagnose various types of cancers and nervous system disorders, such as Alzheimer's, as well as detect drug resistance in infectious diseases. To use the camera it is necessary to first isolate and label the desired DNA with fluorescent tags. Ozcan says such laboratory procedures are possible even in remote locations and resource-limited settings.

To scan the DNA, the group developed a computational interface and Windows smart application running on the same smart phone. The scanned information is then sent to a remote server in Ozcan's laboratory, which measures the length of the DNA molecules. Assuming you have a reliable data connection, the entire data processing takes less than 10 seconds.

In their lab, Ozcan's group tested the device's accuracy by imaging fluorescently labeled and stretched DNA segments. It reliably sized DNA segments of 10,000 base pairs or longer. (A base pair is the basic structural unit of DNA.) Many important genes fall in this size range, including a bacterial gene notorious for giving Staphylococcus aureus and other bacteria antibiotic resistance that is about 14,000 base pairs long.

The smartphone microscope demonstrated a significant drop in accuracy for 5,000 base-pair or shorter segments, however, due to the reduced detection signal-to-noise ratio and contrast for such short fragments. The problem could easily be remedied by replacing the device's current lens with one of a higher numerical aperture, Ozcan said.

In addition to its use in point-of-care diagnostics, Ozcan proposes that his platform could also be useful for differentiating high molecular weight DNA fragments, which are problematic for conventional gel electrophoresis, a frequently used technique in biochemistry and molecular biology to size DNA and RNA fragments. Ozcan's group next plans to test their device in the field to detect the presence of malaria-related drug resistance.


Welcome to SUV System Ltd!

SUV System Ltd is ISO 90012008 Certified electronics distributor with 10 years of experiences.

We have built up long term business relationship with about many companies which are stockers and authorized agents. we have a steady and reliable supply to meet customer's demands to the greatest extent .Confidently, we are able to lower your cost and support your business with our years of professional service.

SUV System Ltd is Electronic Components Distributor Supplies,Find Quality Electronic Components Supplies Products IC(Integrated Circuits),Connectors,Capacitor,Resistors,Diodes,Transistors,LED at Suvsystem.com. Sourcing Other Energy, Environment, Excess Inventory Products from Manufacturers and Suppliers at Suvsystem.com

Electronic Components distributor:http://www.suvsystem.com

Connectors Distributor:http://www.suvsystem.com/l/Connectors-1.html

IC Distributor:http://www.suvsystem.com/l/IC(Integrated-Circuits)-1.html

LED Distributor:http://www.suvsystem.com/l/LED-1.html

Capacitor Distributor:http://www.suvsystem.com/l/Capacitor-1.html

Transistor Distributor:http://www.suvsystem.com/l/Transistors-1.html

Resistor Distributor:http://www.suvsystem.com/l/Resistors-1.html

Diode Distributor:http://www.suvsystem.com/l/Diodes-1.html

SUV System Ltd insists on the managing faith ofsincereness,speciality,foresight, win-win,so we build up stable-relationship customers located all over the world, including the States, Europe, Argentina, UAE, Malaysia, Australia,and India etc

we are focus on the following fields,and hope we can help you.


HARRIS IC LINEAR IC High Precision Resistors Multi-units Transistors LITTELFUSE Diodes MAXIM IC Freescale Semiconductor TOSHIBA Transistors INTERSIL IC Zener Diodes Chip Fuses Ligitek LED BB IC Digital Transistors NEC Diodes IR Diodes ON Diodes Schottky Diodes Switching Diodes components Fast Recovery Diodes ELPIDA IC Current Sensors Resistors Fairchild Semiconductor Transistors Low Ohmic Resistors LED Resistor Arrays TI IC Diodes Inc Chip Ferrite Beads Thin Film Resistors Resistors VISHAY IC PANASONIC Resistors Vishay resistors IR transistor Civil IC YAGEO Resistors Rectifier Diodes DIODES Transistors
http://www.suvsystem.com/a/44636.aspx

No comments:

Post a Comment